Features of the aligned sequences are displayed.
#Vector nti files in serial cloner serial#
During all these sub-cloning steps, Features present in plasmids and insert will be properly inherited.įinally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server and extract a consensus. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. Just select, blunt if you need, and click the Ligate button. You can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. shRNA constructions based on pre-defined scaffolds are also automated. In addition to the classical restriction maps - both graphic and text-based - or site usage windows, you will be able to quickly set-up a PCR-based amplification or prepare synthetic adaptors. Serial Cloner will assist you in setting-up new sub-cloning projects and in preparing the electronic versions of your constructs. Features can be added to protein sequences and inherited from DNA upon translation (done using chosen genetic code). Serial Cloner handles Annotations and Features both in the sequence and in the Graphic Map and can automatically scan for sequence Features. Text-based Sequence maps are also generated and also display Features. The graphic map of Serial Cloner is really Graphic as you can easily select and extract a feature, a fragment or show single, double or multiple cutter all in the same window. Serial Cloner also lets you build text restriction map and quickly format it to add multi-frame translation or only show single cutters for example. All key functions are accessible through a compact interface. All the tools you need to analyze and manipulate your sequences are available in an all-in-one-window concept. Powerful graphical display tools and simple interfaces help the analysis and construction steps in a very intuitive way. Version 2.6 can now estimate the isolectric point of proteins using various methods. Serial Cloner can open protein sequences, align and reverse-translate them using defined codon-biais tables. The included Web interface also allows to submit BLAST request directly from Serial Cloner Sequence windows. Genbank/EMBL files can also be read or imported from internet using the included web interface. Serial Cloner can read and write DNA Strider-compatible files and also import or export files in FASTA format, MacVector, VectorNTI, ApE and GenBank files with their features as well as pDRAW32 sequences. User can annotate any sequence portion or use the automatic scan to detect common features. Serial Cloner 2.6 handles Features/Annotations and allows to define, import and export, customized lists of Features.
#Vector nti files in serial cloner software#
Serial Cloner is a Molecular Biology software that provides tools with an intuitive interface to assists you in DNA cloning, sequence analysis and visualization.
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